5 research outputs found
A Generative Model of Natural Texture Surrogates
Natural images can be viewed as patchworks of different textures, where the
local image statistics is roughly stationary within a small neighborhood but
otherwise varies from region to region. In order to model this variability, we
first applied the parametric texture algorithm of Portilla and Simoncelli to
image patches of 64X64 pixels in a large database of natural images such that
each image patch is then described by 655 texture parameters which specify
certain statistics, such as variances and covariances of wavelet coefficients
or coefficient magnitudes within that patch.
To model the statistics of these texture parameters, we then developed
suitable nonlinear transformations of the parameters that allowed us to fit
their joint statistics with a multivariate Gaussian distribution. We find that
the first 200 principal components contain more than 99% of the variance and
are sufficient to generate textures that are perceptually extremely close to
those generated with all 655 components. We demonstrate the usefulness of the
model in several ways: (1) We sample ensembles of texture patches that can be
directly compared to samples of patches from the natural image database and can
to a high degree reproduce their perceptual appearance. (2) We further
developed an image compression algorithm which generates surprisingly accurate
images at bit rates as low as 0.14 bits/pixel. Finally, (3) We demonstrate how
our approach can be used for an efficient and objective evaluation of samples
generated with probabilistic models of natural images.Comment: 34 pages, 9 figure
Molecular structures of unbound and transcribing RNA polymerase III
Transcription of genes encoding small structured RNAs such as tRNAs, spliceosomal U6 snRNA and ribosomal 5S RNA is carried out by RNA polymerase III (Pol III), the largest yet structurally least characterized eukaryotic RNA polymerase. The cryo-EM structures of the S. cerevisiae Pol III elongating complex at 3.9 Ă… resolution and the apo Pol III enzyme in two different conformations at 4.6 and 4.7 Ă… resolution, respectively, allow for the first time to build a 17-subunit atomic model of Pol III. The reconstructions reveal the precise orientation of the C82/C34/C31 heterotrimer in close proximity to the stalk. The C53/C37 heterodimer positions residues involved in transcription termination close to the non-template DNA strand. In the apo Pol III structures, the stalk adopts different orientations coupled with closed and open conformations of the clamp. Our results provide novel insights into Pol III-specific transcription and the adaptation of Pol III towards its small transcriptional targets